Hab ay of tallaabo of falgalka

Falgalka (ensaymes) - a kelli miisaanka sare xeryahooda organic dabiiciga ah protein, kaas oo ka shaqeeya u shaqeeyo door ah Xoojiya noolaha.

hab ay of tallaabo of falgalka

Aayahiisu hababka salka tallaabada horseed of falgalka, waa mid ka mid ah dhibaatooyinka asaasiga ah iyo dhibaatooyinka hadda enzymology ma aha oo kaliya, laakiin Kiimikada noole casriga ah iyo biology kelli.

Long ka hor inta ensaymes noqday heli saafi ah, oo waxaa elucidated ay dabiicadda, ayaa noqotay qanacsanahay in ay muhiim u tahay geedi socodka enzymatic ayaa xigay ka mid ah enzyme in substrate ah. Isku dayaysa in ay ka heli xarunta adag ee enzyme la substrate ee waqti dheer ma noqonin mid horseeda in guusha, sida adag tani waa Labile, waxa aad si degdeg ah decomposes. Isticmaalka habka spectroscopy ayaa ka dhigtay in ay suurto gal ah in la aqoonsado kaxeynayo enzyme-substrate si catalase, perodoxida, dehydrogenase khamriga, falgalka flavinzavisimyh.

Habka of Falanqaynta X-ray ayaa la oggol yahay si aad u hesho macluumaad badan oo muhiim ah oo ku saabsan qaab dhismeedka iyo habab horseed garoomada of falgalka. Habka waxa loo adeegsaday in la dhiso a analogues substrate xidhiidh la leh ensaymes lysozyme iyo chymotrypsin.

Qaar ka mid ah caddayn toos ah ee jiritaanka kaxeynayo enzyme-substrate suurtagashay in ay hesho halka mid ka mid ah marxaladaha wareegga horseed ee eulayska ku xidhan tahay in substrate ah by bond covalent ah. Tusaale waxaa ah falcelinta ee hydrolysis of copolymer n-nitrophenyl catalyzed by chymotrypsin. Marka lagu qaso ether chymotrypsin enzyme la acetylated ee group hydroxyl ka hadhayna serine Waxyeelo ah. stage Tani waa si degdeg ah, laakiin hydrolysis atsetilhimotripsina la formation of chymotrypsin copolymer oo lacag la'aan ah waa badan gaabis ah. Sidaa darteed atsetilhimotripsin ururayaa, kaas oo si fudud u ogaan in joogitaanka n-nitrophenylacetate.

Joogitaanka substrate enzyme ee ka kooban karo "qabsado" by adag deganayn kala iibsiga EU ee foomka firfircoonayn, tusaale ahaan, by daaweynta adag enzyme-substrate ee borohydride sodium isagoo saamayn yaraynta xoog ah. adag oo ah in qaab derivative covalent deggan noocan oo kale ah lagu arkay dalka aldolase eulayska. Waxa la ogaaday in substrate ah ula dhaqmo koox Britain e-amino ee lysine.

substrate The sameynayso la enzyme ee qayb gaar ah oo la yidhaahdo xarunta firfircoon ee enzyme ama aagga firfircoon.

Under xarunta firfircoon, ama muhiimka ah, fahamsanahay in enzyme qayb Britain protein oo ku xiran in substrate ah (iyo cofactors) oo keena hantida enzymatic ee Britain ayaa ku. Goobta firfircoon go'aamisa koobnaanta iyo dhaqdhaqaaqa fikraan of enzyme iyo waa in ay shahaado qaar ka mid ah qaab-dhismeedka dhib loogu talo galay hab dhow oo la macaamilaan Britain substrate ah, ama qaybo ka mid ay si toos ah oo ku lug leh dareen-celinta.

Waxaa ka mid ah kooxaha functional waxaa kala duwanaayeen ka mid ah "catalytically firfircoon" qayb ka mid ah eulayska iyo qaybtii sameynta bixinta buuna gaar ah (ku qabanaya of substrate si eulayska) - pin waxa loogu yeero, ama "baroosin" (ama adsorption of enzyme goobta firfircoon).

hab ay of tallaabo of falgalka sharxayaa aragtida ah oo ka mid ah isla'egta Michaelis-Menten. Sida laga soo xigtay aragtida this, geeddi-socodka meel afar marxaladood.

hab ay of tallaabo of falgalka: Waxaan Phase

Inta u dhaxaysa substrate ah (C) iyo enzyme (E) xiriir baxdo - sameeyay adag enzyme-substrate EU, kuna waari dhexdeeda qaybood waxaa isku xidhan by covalent, Madbacada qaranka, biyaha iyo dhaaraheedii kale.

hab ay of tallaabo of falgalka: stage IІ

substrate waxaa bandhigi enzyme ku lifaaqan waxaa firfircoonaan iyo noqdo diyaar u ah dhibaatooyinka kala duwan ee catalysis Midowga Yurub.

hab ay of tallaabo of falgalka: stage IІI

EU go'an catalysis. Aragtida sidaan ah waxaa lagu taageeray by cilmiga tijaabo ah.

Ugu dambeyntii, stage IV waxa lagu gartaa sii daayo ee u taagoo eulayska E iyo F. isku xigxiga ee transformations badeecada lagu soo bandhigi karaa sida dareen-celinta: E + S - EU EU - * - E + P.

cayimayo ficilka enzyme

enzyme kasta oo u dhaqmo on substrate gaar ah ama koox walxaha, kuwaas oo la mid ah in qaab-dhismeedka. Cayimayo dhaqdhaqaaqa enzymatic ay sabab u tahay isku mid ah oo qaabeynta goobta firfircoon iyo substrate. Inta lagu guda jiro dhexgalka ee enzyme-substrate sameeyay adag.